Escherichia coli - GFP Core Production System

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A Biomanufacturing laboratory manual focusing on one of three NBC2 Core Production Systems utilizing recombinant cells to make proteins-of-interest. This manual focuses on Green Fluorescent Protein (GFP) producing Escherichia coli cells. This laboratory manual is meant for use in introductory biotechnology courses at the high school and community college levels. It covers hands-on equipment and process SOPs for the production of GFP within E. coli. Students will learn basic laboratory skills such as micropipetting, media and buffer preparation, and serial dilutions. This laboratory manual was created and tested by the Northeast Biomanufacturing Center and Collaborative. This NSF ATE funded project is dedicated to building infrastructure for Biomanufacturing education and training, and the workforce.