Protein Is Cash 2014

June, 2014



June 9-13 Glendale Community College, Glendale, AZ
June 22-23 Bucks County Community College, Newtown, PA
July 21-25 Bergen Community College, Paramus, NJ
August 4-8 Texas A & M University, College Station TX


2014 PROTEIN IS CASH LAB MANUAL (Click to Download)


Day One ~ Facilities and Metrology
Power Point Overview:     So, What Is Biomanufacturing?
Power Point Laboratory:     Metrology Instrumentation and Its Limits
Power Point Laboratory:     Pipette Calibration
Video:     Mike Cicio, Bench Top to Bottle
Article 1:     Biotech Set To Dominate Drug Industry Growth, Evaluate Pharma, June 17, 2009 
Article 2:     Biotech, The Community Way, Nature, Volume 458, March 2009
Executive Summary:     National Bioeconomy Blueprint, April 2012

Day Two ~ Transformation and Upstream Processing
Power Point Overview:     Discovery Research and Cell Culture
Power Point Laboratory:     pGLO Bacterial Transformtion Kit
Pre-Lab Activities from Christie Caldwell, Hebron High School, Lewisville, TC (Help Students Understand Sequence of Processes and Tools Used in pGLO Transformation):
Lab Instructions
Process Cards
Tool Cards
Video:     Tim Charlebois, Technology and Opportunities in Mammalian Cell Culture
Article 1:     Arnold Demain, Microbial Biotechnology
Article 2:     Arnold Demain, Small Bugs Big Business: The Economic Power of the Microbe
Article 3:     Tilman Gerngross, Therapeutics in Yeast
Article 4:     GlycoFi, Scientists Report Full Humanization of Yeast Glycosylation Pathway
Article 5:     Florian M Wurm, Therapeutics in Animal Cells
Article 6:     Ian Freshney, Culture of Animal Cells - Scale Up
Article 7:     Kristof De Schutter et al, Genome sequence of the recombinant protein production host Pichia pastoris
Article 8:     A. Varki et al, Essentials of glycobiology
Article 9:     David Cyranoski, Stem-cell Pioneer Banks On Future Therapies
Article 10:     BioPharm International, Applying Fusion Protein Technology to E. coli
Image 1:     Pichia pastoris
Image 2:     Escherichia coli
Image 3:     CHO Cells
Animation:     Inner Life of the Cell

Day Three ~ Downstream Processing
Power Point Overview:     Downstream Processing in Biopharmaceutical Manufacturing
Power Point Laboratory:     Introduction to GFP Chromatography
Video:     GFP Chromatography
Virtual Laboratory:     Virtual Chromatography Laboratory (to login enter your email address and the password: teachbio)
Article 1:     GFP The green fluorescent protein: discovery, expression and development (Nobel Address 2008)
Article 2:     Purification by Liquid Chromatography
Article 3:     Green fluorescent protein: Vibrations Key to Efficiency of GFP
Animation:     Column Chromatography
Order Free Poster:     Fluorescent Proteins Illuminate Cell Biology
or View and Print Poster
and View and Print Supplementary Information
Web Link:     Examining GFP, In Cyberspace and Real Space
Web Link:     Green Fluorecent Protein

Day Four ~ Quality Control Biochemistry
Power Point Overview:     Quality Control Biochemistry
Power Point Overview:     PAGE Electrophoresis

Day Five ~ Biofuels: A Crossover Industry
Power Point Overview:     Biofuels Production & Analysis
Power Point Laboratory:     Biofuel Industry
Power Point Overview:     Biofuels Enzyme Kit
Educational Resources:     Damon Tighe's Dropbox Address for Bio-Rad's Cellulosic Biofuels Kit
Article:     US Biogas Industry Continues to Innovate in the Face of Adversity

Appendix: Biotechnology/Biomanufacturing Careers
ATV TV Video 1:     What Is Biomanufacturing?
ATV TV Video 2:     Student Profiles
ATV TV Video 3:     Products From Living Cells
ATV TV Video 4:     What Is An SOP?
ATV TV Video 5:     Community College Programs
ATV TV Video 6:     Influencing Quality of Life


Location Activities: 
Monday, June 9, 2014 to Friday, June 13, 2014
Monday, July 21, 2014 to Friday, July 25, 2014
Monday, August 4, 2014 to Friday, August 8, 2014